Omentin-1 inhibits the development of benign prostatic hyperplasia by attenuating local inflammation.

BACKGROUND: Benign prostatic hyperplasia (BPH) is a prevalent disease affecting elderly men, with chronic inflammation being a critical factor in its development. Omentin-1, also known as intelectin-1 (ITLN-1), is an anti-inflammatory protein primarily found in the epithelial cells of the small intestine. This study aimed to investigate the potential of ITLN-1 in mitigating BPH by modulating local inflammation in the prostate gland. METHODS: Our investigation involved two in vivo experimental models. Firstly, ITLN-1 knockout mice (Itln-1-/-) were used to study the absence of ITLN-1 in BPH development. Secondly, a testosterone propionate (TP)-induced BPH mouse model was treated with an ITLN-1 overexpressing adenovirus. We assessed BPH severity using prostate weight index and histological analysis, including H&E staining, immunohistochemistry, and enzyme-linked immunosorbent assay. In vitro, the impact of ITLN-1 on BPH-1 cell proliferation and inflammatory response was evaluated using cell proliferation assays and enzyme-linked immunosorbent assay. RESULTS: In vivo, Itln-1-/- mice exhibited elevated prostate weight index, enlarged lumen area, and higher TNF-α levels compared to wild-type littermates. In contrast, ITLN-1 overexpression in TP-induced BPH mice resulted in reduced prostate weight index, lumen area, and TNF-α levels. In vitro studies indicated that ITLN-1 suppressed the proliferation of prostate epithelial cells and reduced TNF-α production in macrophages, suggesting a mechanism involving the inhibition of macrophage-mediated inflammation. CONCLUSION: The study demonstrates that ITLN-1 plays a significant role in inhibiting the development of BPH by reducing local inflammation in the prostate gland. These findings highlight the potential of ITLN-1 as a therapeutic target in the management of BPH.

The inflammatory cytokine profiles and ocular biometric characteristics of primary angle-closure glaucoma.

OBJECTIVE: To investigate the pathogenesis of primary angle-closure disease (PACG) by measuring the anatomical structures of the anterior and posterior segments of the eye and inflammatory markers in the peripheral blood. METHODS: This case-control study enrolled patients diagnosed with acute PACG (APACG) and chronic PACG (CPACG). It also enrolled control subjects without PACG. The anterior and posterior anatomical features were measured in all study participants. The levels of interleukin (IL)-6, tumour necrosis factor-α and the neutrophil-to-lymphocyte ratio (NLR) in the peripheral blood were measured. RESULTS: This study analysed a total of 99 eyes: 34 eyes from 34 patients with APACG, 28 eyes from 28 patients with CPACG and 37 eyes from 37 control patients with senile cataract. The axis length, corneal diameter, anterior chamber depth and anterior chamber volume were significantly smaller in the APACG and CPACG groups compared with the controls. The level of IL-6 in the peripheral blood of patients with PACG was significantly lower than that of the controls. The NLR in the peripheral blood of patients with PACG was significantly greater than that of the controls. CONCLUSIONS: Changes in the ocular anatomy and some inflammatory markers might be involved in the pathogenesis of PACG.

Interactions between micro-scale oil droplets in aqueous surfactant solution determined using optical tweezers.

HYPOTHESIS: The stability of the emulsions is crucial, which relies on a well-developed understanding of dynamic interaction forces between single dispersed droplets. In the previous studies, many interests focus on the oil droplets of size range of 20-200 µm. However, emulsion droplets with diameter below 10 µm are rarely mentioned, which is the size scale of real emulsion droplets in various applications, such as toners, spacers for liquid crystal displays, and materials in biomedical and biochemical analysis. The micro-scale droplets have many differences on the deformation, internal pressure and hydrodynamic effects. It is necessary to understand the interaction mechanisms between two real size scales of oil droplets for guiding practical production and application. EXPERIMENTS: In this work, tetradecane was chosen as the model oil phase in all experiments. The interaction forces of two tetradecane droplets with the diameter of 5.0 µm in water in the presence of surfactant and salt solution were directly measured using optical tweezers. The force-distance curves were established, and the zeta potential of tetradecane droplets was studied using Zetasizer Nano ZSP. FINDINGS: The absolute value of zeta potential of tetradecane droplets was found to decrease with the increase of salt concentration and increase with the increase of surfactant concentration. The repulsive force between two tetradecane droplets was found to decrease with the increase of salt concentration because the electrostatic double-layer force was suppressed gradually with the increase of salt concentration. The "hydrodynamic suction" effect during the process of retraction becomes more pronounced due to the corresponding increase in the hydrodynamic force with the increase of the approaching velocity between the tetradecane droplets. Furthermore, we found the existing model for the measurement of large droplets by atomic force microscope (AFM) is invalid for the measurement of micro-scale droplets by optical tweezers. The deformation of colliding micro-scale droplets can be safely ignored, which is quite different from the large droplets. Our results provide a useful method to study the interaction forces between micro-scale emulsion droplets with pN force resolution, and gives a deep insight of the stabilization mechanism of real size scale of O/W emulsions. These findings have significant implications on the stability of emulsions in many food, cosmetics, medicine, and advanced materials.

Temperature- and pH-sensitive core-shell nanoparticles self-assembled from poly(n-isopropylacrylamide-co-acrylic acid-co-cholesteryl acrylate) for intracellular delivery of anticancer drugs.

Temperature- and pH-sensitive amphiphilic polymer poly(N-isopropylacrylamide-co-acrylic acid-co-cholesteryl acrylate) (P(NIPAAm-co-AA-co-CHA)) has been synthesized and employed to encapsulate paclitaxel, a highly hydrophobic anticancer drug, in core-shell nanoparticles fabricated by a membrane dialysis method. The nanoparticles are spherical in shape, and their size can be made below 200 nm by varying fabrication parameters. The lower critical solution temperature (LCST) of the nanoparticles is pH-dependent. Under the normal physiological condition (pH 7.4), the LCST is well above the normal body temperature (37 degrees C) but it is below 37 degrees C in an acidic environment (e.g. inside the endosome or lysosome). The critical association concentration of the polymer is determined to be 7 mg/L. Paclitaxel can be easily encapsulated into the nanoparticles. Its encapsulation efficiency is affected by fabrication temperature, initial drug loading and polymer concentration. In vitro release of paclitaxel from the nanoparticles is responsive to external pH changes, which is faster in a lower pH environment. Cytotoxicity of paclitaxel-loaded nanoparticles against MDA-MB-435S human breast carcinoma cells is slightly higher than that of free paclitaxel. In addition, doxorubicin is used as a probe to study cellular uptake using a confocal laser scanning microscope (CLSM). Doxorubicin molecules are able to enter the cytoplasm after escaping from the endosome and/or the lysosome. The temperature- and pH-sensitive nanoparticles would make a promising carrier for intracellular delivery of anticancer drugs.